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. 1988 May 25;16(10):4679–4692. doi: 10.1093/nar/16.10.4679

Complex promoter pattern of the single ribosomal RNA operon of an archaebacterium Halobacterium halobium.

A S Mankin 1, V K Kagramanova 1
PMCID: PMC336657  PMID: 3380693

Abstract

The transcription of the single ribosomal RNA gene cluster is initiated at an assemblage of promoters five of which were previously mapped in the 910 base pair region in front of the 16S rRNA gene. The nucleotide sequence between positions -1680 and -910 was determined and S1 nuclease mapping revealed therein two sites corresponding to the 5' ends of the primary transcripts. These two presumably represent the most distal promoters, P1 and P2, of the operon, P1 being separated from the 16S rRNA gene by more than 1250 bp. An additional putative "internal" promoter located within the operon body in the 16S/23S intergenic spacer was revealed by S1 nuclease mapping. A 528 bp long open reading frame is co-transcribed with the rRNA genes from the P1 promoter. Its possible functional implication is discussed.

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Selected References

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  1. Boros I., Csordás-Tóth E., Kiss A., Kiss I., Török I., Udvardy A., Udvardy K., Venetianer P. Identification of two new promoters probably involved in the transcription of a ribosomal RNA gene of Escherichia coli. Biochim Biophys Acta. 1983 Mar 10;739(2):173–180. doi: 10.1016/0167-4781(83)90027-1. [DOI] [PubMed] [Google Scholar]
  2. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol. 1981 May 15;148(2):107–127. doi: 10.1016/0022-2836(81)90508-8. [DOI] [PubMed] [Google Scholar]
  3. Chant J., Dennis P. Archaebacteria: transcription and processing of ribosomal RNA sequences in Halobacterium cutirubrum. EMBO J. 1986 May;5(5):1091–1097. doi: 10.1002/j.1460-2075.1986.tb04327.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daniels C. J., Gupta R., Doolittle W. F. Transcription and excision of a large intron in the tRNATrp gene of an archaebacterium, Halobacterium volcanii. J Biol Chem. 1985 Mar 10;260(5):3132–3134. [PubMed] [Google Scholar]
  5. Dennis P. P. Multiple promoters for the transcription of the ribosomal RNA gene cluster in Halobacterium cutirubrum. J Mol Biol. 1985 Nov 20;186(2):457–461. doi: 10.1016/0022-2836(85)90117-2. [DOI] [PubMed] [Google Scholar]
  6. Dunn R., McCoy J., Simsek M., Majumdar A., Chang S. H., Rajbhandary U. L., Khorana H. G. The bacteriorhodopsin gene. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6744–6748. doi: 10.1073/pnas.78.11.6744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
  8. Hentschel C., Irminger J. C., Bucher P., Birnstiel M. L. Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences. Nature. 1980 May 15;285(5761):147–151. doi: 10.1038/285147a0. [DOI] [PubMed] [Google Scholar]
  9. Kagramanova V. K., Mankin A. S., Baratova L. A., Bogdanov A. A. The 3'-terminal nucleotide sequence of the Halobacterium halobium 16 S rRNA. FEBS Lett. 1982 Jul 19;144(1):177–180. doi: 10.1016/0014-5793(82)80595-4. [DOI] [PubMed] [Google Scholar]
  10. Mankin A. S., Skripkin E. A., Kagramanova V. K. A putative internal promoter in the 16 S/23 S intergenic spacer of the rRNA operon of archaebacteria and eubacteria. FEBS Lett. 1987 Jul 27;219(2):269–273. doi: 10.1016/0014-5793(87)80233-8. [DOI] [PubMed] [Google Scholar]
  11. Mankin A. S., Teterina N. L., Rubtsov P. M., Baratova L. A., Kagramanova V. K. Putative promoter region of rRNA operon from archaebacterium Halobacterium halobium. Nucleic Acids Res. 1984 Aug 24;12(16):6537–6546. doi: 10.1093/nar/12.16.6537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  13. Moritz A., Lankat-Buttgereit B., Gross H. J., Goebel W. Common structural features of the genes for two stable RNAs from Halobacterium halobium. Nucleic Acids Res. 1985 Jan 11;13(1):31–43. doi: 10.1093/nar/13.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moss T., Birnstiel M. L. The putative promoter of a Xenopus laevis ribosomal gene is reduplicated. Nucleic Acids Res. 1979 Aug 24;6(12):3733–3743. doi: 10.1093/nar/6.12.3733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mount D. W., Conrad B. Improved programs for DNA and protein sequence analysis on the IBM personal computer and other standard computer systems. Nucleic Acids Res. 1986 Jan 10;14(1):443–454. doi: 10.1093/nar/14.1.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reiter W. D., Palm P., Voos W., Kaniecki J., Grampp B., Schulz W., Zillig W. Putative promoter elements for the ribosomal RNA genes of the thermoacidophilic archaebacterium Sulfolobus sp. strain B12. Nucleic Acids Res. 1987 Jul 24;15(14):5581–5595. doi: 10.1093/nar/15.14.5581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Travers A. A. Conserved features of coordinately regulated E. coli promoters. Nucleic Acids Res. 1984 Mar 26;12(6):2605–2618. doi: 10.1093/nar/12.6.2605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wich G., Hummel H., Jarsch M., Bär U., Böck A. Transcription signals for stable RNA genes in Methanococcus. Nucleic Acids Res. 1986 Mar 25;14(6):2459–2479. doi: 10.1093/nar/14.6.2459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wich G., Jarsch M., Böck A. Apparent operon for a 5S ribosomal RNA gene and for tRNA genes in the archaebacterium Methanococcus vannielii. Mol Gen Genet. 1984;196(1):146–151. doi: 10.1007/BF00334107. [DOI] [PubMed] [Google Scholar]
  20. Wich G., Leinfelder W., Böck A. Genes for stable RNA in the extreme thermophile Thermoproteus tenax: introns and transcription signals. EMBO J. 1987 Feb;6(2):523–528. doi: 10.1002/j.1460-2075.1987.tb04784.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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