TABLE I.

Experimental parameters for the working fluids used in this study.

Experimental parameter	Pure water	in vitro blood
Height × width × length of the microchannel before bifurcation	160 ± 1 μm × 50 ± 1 μm × 40mm	160 ± 1 μm × 50 ± 1 μm × 40mm
Flow rate	3.0 × 10−12 m3/s	3.3 × 10−12 m3/s
Mean velocity in the inlet channel	0.38 mm/s	0.41 mm/s
Particle/RBC diameter at rest	1 μm	≈ 8 μm
Particle/RBC concentration	0.1%	14%
Dextran 40 concentration	—	≈ 9%
Viscosity of the suspending fluid	7.1 × 10−4 Pa·s	4.5 × 10−3 Pa·s
Temperature	37°C	37°C
Magnification (M)	32×	32×
Numerical aperture (NA)	0.75	0.75
λex excitation wavelength	532 nm	532 nm
Refractive index of PDMS (n)	1.4	1.4
Optical slice thickness (OST)a	6.94 μm	6.94 μm
Capture image rate	100 frames/s	100 frames/s

^aA detailed description to calculate the OST can be found in Lima et al.¹⁰