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. 1992 Jun 11;20(11):2773–2776. doi: 10.1093/nar/20.11.2773

The influence of single base triplet changes on the stability of a pur.pur.pyr triple helix determined by affinity cleaving.

P A Beal 1, P B Dervan 1
PMCID: PMC336921  PMID: 1614865

Abstract

The influence of sixteen base triplet changes at a single position within a pur.pur.pyr triple helix was examined by affinity cleaving. For the 15 base pair target site studied here, G.GC, A.AT and T.AT triplets stabilize a triple helix to a greater extent than the other 13 natural triplets (pH = 7.4, 25 degrees C). Weaker interactions were detected for the C.AT, A.GC and T.CG triplets. The absence of specific, highly stabilizing interactions between third strand bases and the CG or TA base pairs demonstrates a current sequence limitation to formation of this structure. Models for the two dimensional base triplet interactions for all possible 16 natural triplets are presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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