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. 2012 Apr 20;287(24):20443–20455. doi: 10.1074/jbc.M112.362087

FIGURE 2.

FIGURE 2.

Effect of the MyrARF6 and MyrARF1 inhibitory peptides on HCG-stimulated HLHCGR internalization and cAMP accumulation. A, HEK-HLHCGR cells were incubated with 0.1–5 μm penetratin (■), penetratin-coupled Myr-ARF1 (●) or penetratin-coupled Myr-ARF6 (▴) peptides for 2 h at 37 °C and then incubated in the presence or absence of 10 IU/ml HCG for 30 min. HLHCGR internalization was determined by ELISA. B, immunofluorescence analysis showing the HCG-induced (10 IU/ml HCG, 30 min) internalization of HLHCGR cells in the absence or presence of 2.5 μm penetratin or penetratin coupled Myr-ARF1 or Myr-ARF6 peptides. C, cAMP accumulation in HCG-stimulated (10 IU/ml HCG, 30 min) HEK-HLHCGR cells in the presence of 0.1–5 μm penetratin, penetratin-coupled Myr-ARF1, or penetratin-coupled Myr-ARF6 peptides.