Abstract
Pseudomonas mandelii is a cold-adapted bacterium that can grow at 4°C but not at 37°C. Here we report the draft genome sequence of P. mandelii strain JR-1.
GENOME ANNOUNCEMENT
Pseudomonas mandelii exhibits nonhalophilic characteristics and flourishes at low temperatures, producing extracellular enzymes (3). However, it does not grow at 37°C (3). P. mandelii is phylogenetically closely related to Pseudomonas fluorescens (5). P. mandelii has been reported to occur in natural mineral waters (6) and agricultural fields (2, 7).
Pseudomonas mandelii strain JR-1 was isolated from natural mineral waters in Gyeongsan, South Korea (4). Its optimum growth temperature was 25 to 30°C (4). It grew at 4°C but not at 37°C, like a previously reported P. mandelii strain (3). P. mandelii JR-1 secreted extracellular lipolytic enzymes (4).
The draft genome sequence of P. mandelii JR-1 was determined using a Roche GS-FLX 454 pyrosequencing system (Macrogen, South Korea). A total of 822,189 reads (263,370,094 nucleotides) were generated, reaching a depth of 37-fold genome coverage. Using the GS de novo Assembler software (version 2.6), they were assembled into 185 contigs, of which 96 were larger than 500 bp. The draft genome of P. mandelii JR-1 is approximately 7.1 Mb, with an average G+C content of 57.9%. The open reading frames in its genome were predicted using the RAST (Rapid Annotation using Subsystem Technology) server (1) to include 6,374 protein-coding genes and 54 tRNA and 5 rRNA genes. The P. mandelii JR-1 genome exhibited high homology to the genomes of Pseudomonas fluorescens strains Pf0-1, Pf-5, and SBW25.
Nucleotide sequence accession number.
The draft genome sequence of P. mandelii JR-1 (96 large contigs) has been assigned GenBank accession no. AJFM00000000.1.
ACKNOWLEDGMENT
This work was supported by the Daegu University Research Grant, 2009 (to C.L.).
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