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. Author manuscript; available in PMC: 2013 Jul 7.
Published in final edited form as: Analyst. 2012 Feb 8;137(13):2997–3003. doi: 10.1039/c2an16173g

Figure 5.

Figure 5

Autocorrelation functions of representative blank Ramos cell (A), control Ramos cell stained with 0.2 µM 2SBPO-Casp(B), and a Ramos cell induced with 1.75 µg/ml staurosporine and incubated for 3 h (C). The fluorescence signal is uncorrelated in both the blank and control cells, while apoptotic cells show a correlated fluorescence. Photon counting histograms (D) of blank Ramos cell (black), control Ramos cell stained with 0.2 µM 2SBPO-Casp(red), and a Ramos cell induced with 1.75 µg/mL staurosporine and incubated for 3 h (blue) show the intensity differences can also be used to distinguish apoptotic cells.