Fig. 1.

Method of integrating epidemiological and toxicological data to create variant by environment interaction candidates. (A) Summary of epidemiological databases, VARIMED (source of genetic associations, red) and NHANES (source of environmental data, green). (B) Factors are chosen by their strength of association to T2D depicted by Manhattan plots from VARIMED or EWAS process with NHANES. A threshold p and q are chosen to choose candidate factors (examples in italics). (C) Variants are mapped to gene promoter, exon or intron; environmental factors are mapped to MeSH ID. (D) Factors are queried for curated evidence regarding molecular-level interaction in CTD. Knowledge is coded in CTD in an example on right. (E) Presence of interacting pair in CTD is a candidate for further study in context of T2D