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. 2012 May;136(1):54–63. doi: 10.1111/j.1365-2567.2012.03557.x

Figure 4.

Figure 4

In vitro activation of mutant B cells. (a) Evaluation by ELISA of in vitro IgA secretion after lipopolysaccharide (LPS) plus transforming growth factor- β (TGF-β) stimulation during 4 days of splenocytes from αΔtail+/+ mutant mice compared with wild-type (wt). (b) Semi-quantitative RT-PCR experiments were performed on RNA harvested at day 3 from unstimulated and in vitro-stimulated B cells with LPS plus TGF-β. Serial dilutions of template cDNA are performed at 1 : 1, 1 : 5, 1 : 25, and 1 : 125 for all transcripts. Germline (Iμ-Cμ, Iα-Cα) and class-switched (Iμ-Cα) transcripts were analysed on such serial dilutions. Transcription of the β-actin gene was used as a control.