Figure 3. Deficiency of TGF-β1 in activated CD4+ T cells and Treg cells inhibits tumor development (A) Tgfb1f/n-TRAMP and Tgfb1f/nTnfrsf4-cre-TRAMP mice were evaluated for tumor development at 8 months of age. The weights (Wt) of urogenital tracts (UG) normalized to body Wt ± s.e.m of Tgfb1f/n TRAMP (n = 4) and Tgfb1f/nTnfrsf4-cre TRAMP (n = 3) mice are shown. The p value between two groups of tumor burden is shown (Students t-test). *Depicts statistically significant difference. (B) Flow cytometric analysis examining the expression of interferon gamma (IFNγ), granzyme B (GzmB) and PD-1 proteins in CD4+ and CD8+ T cells in the draining lymph nodes and prostates of Tgfb1f/n TRAMP and Tgfb1f/nTnfrsf4cre-TRAMP mice. For IFNγ expression, T cells were re-stimulated in vitro for five hours with phorbol 12-myristate 13-acetate (PMA), ionomycin and GolgiStop. Representatives of two independent experiments are shown.