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. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: Science. 2012 Mar 30;335(6076):1617–1621. doi: 10.1126/science.1212476

Figure 3. Reduced homeostasis and decreased lifespan in elav>CycA-RNAi animals.

Figure 3

(A) Delayed sleep onset in elav>CycA-RNAi animals (n=80) compared to controls [elavGal4 (n=64), UAS-CycA-RNAi (n=36)] after lights went off. (B, C) Decreased recovery of sleep after overnight sleep deprivation in elav>CycA-RNAi animals (n=27), compared to controls [elavGal4 (n=52), UAS-CycA-RNAi (n=10)]. Sleep lost (B) was calculated by subtracting sleep on the night of the deprivation from undisturbed sleep the night before. All animals analyzed lost at least 95% of their sleep. To account for the differences in baseline sleep between the genotypes, recovery data (C) were normalized. (D) Reduced lifespan in elav>CycA-RNAi (n=113) animals compared to controls [elavGal4 (n=102), UAS-CycA-RNAi (n=100)]. Statistical analysis of sleep was performed using One-way ANOVAs with Tukey post-test, and statistical analysis of longevity was performed using Log-rank tests. In all experiments described in this figure, elavGal4 drives the expression of UAS-Dicer2.