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. 1988 Mar 11;16(5 Pt A):1693–1702. doi: 10.1093/nar/16.5.1693

The design of synthetic genes.

S R Presnell 1, S A Benner 1
PMCID: PMC338164  PMID: 2451218

Abstract

Computer programs are described that aid in the design of synthetic genes coding for proteins that are targets of a research program in site directed mutagenesis. These programs "reverse-translate" protein sequences into general nucleic acid sequences (those where codons have not yet been selected), map restriction sites into general DNA sequences, identify points in the synthetic gene where unique restriction sites can be introduced, and assist in the design of genes coding for hybrids and evolutionary intermediates between homologous proteins. Application of these programs therefore facilitates the use of modular mutagenesis to create variants of proteins, and the implementation of evolutionary guidance as a strategy for selecting mutants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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