Abstract
Software has been developed to assist RNA fingerprinting analysis. One program generates, from a DNA sequence data file, the oligonucleotides resulting from digestion of an RNA transcript labeled with any specified nucleotide(s). Oligonucleotides are sorted according to their position on the fingerprint. Expected molar yields and products of secondary redigestion are also indicated. A second program facilitates calculation of experimental molar yields of oligonucleotides.
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Selected References
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- Engelke D. R., Gegenheimer P., Abelson J. Nucleolytic processing of a tRNAArg-tRNAAsp dimeric precursor by a homologous component from Saccharomyces cerevisiae. J Biol Chem. 1985 Jan 25;260(2):1271–1279. [PubMed] [Google Scholar]
- Gegenheimer P., Apirion D. Structural characterization and in vitro processing of Escherichia coli ribosomal RNA transcripts containing 5- triphosphates, leader sequences, 16 S rRNA, and spacer tRNAs. J Mol Biol. 1980 Nov 5;143(3):227–257. doi: 10.1016/0022-2836(80)90188-6. [DOI] [PubMed] [Google Scholar]
- Gegenheimer P., Gabius H. J., Peebles C. L., Abelson J. An RNA ligase from wheat germ which participates in transfer RNA splicing in vitro. J Biol Chem. 1983 Jul 10;258(13):8365–8373. [PubMed] [Google Scholar]