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. Author manuscript; available in PMC: 2013 Aug 7.
Published in final edited form as: Oncogene. 2012 Mar 19;32(6):759–767. doi: 10.1038/onc.2012.90

Figure 3. siYES and siLYN can reduce the nuclear localization of the EGFR.

Figure 3

(A) siYES and siLYN decreased phosphorylation of EGFR tyrosine 845 (Y845), tyrosine 1101 (Y1101) and SFK tyrosine 419. Cells were harvested 72 hr after treatment with either siYES or siLYN, and protein lysates were fractionated on SDS-PAGE followed by immunoblotting for the indicated proteins. The non-targeting siRNA (NT) was used as a control. α-tubulin was used as a loading control. Protein expression was quantitated using ImageJ software. (B) siYES and siLYN can reduce the nuclear localization of the EGFR. Cells were harvested for non-nuclear and nuclear protein, and fractionated on SDS-PAGE followed by immunoblotting for indicated proteins after 72hr treatment with either siYES or siLYN. The non-targeting siRNA (NT) was used as a control. Histone H3 and α-tubulin were used as loading and purity controls for the nuclear and non-nuclear fractions, respectively. Protein expression was quantitated using ImageJ software.