Figure 4. YES and LYN can induce the nuclear localization of the EGFR.

(A) Increased levels of total protein expression of Yes and Lyn were detected in Chinese hamster ovary (CHO-K1) cells post 48hr transfection with either a Yes or Lyn mammalian expression vector. (B) Increased Yes and Lyn expression as well as increased levels of pSFK Y419 were observed in Ctx^S cells after 48hr transfection compared to vector only. (C) Nuclear EGFR translocation was increased by overexpression of Yes and Lyn. Cells were transfected with a Yes or Lyn mammalian expression vector and harvested for non-nuclear and nuclear protein. Each protein was fractionated on SDS-PAGE followed by immunoblotting. Histone H3 and α-tubulin were used as loading and purity controls for the nuclear and non-nuclear fractions, respectively. (D) Ctx^R clones (HC1, HC4 and HC8) have increased nuclear Yes and Lyn localization as compared to Ctx^s cell line (HP). Cells were harvested for non-nuclear and nuclear protein, and fractionated on SDS-PAGE followed by immunoblotting for indicated proteins. Histone H3 and α-tubulin were used as loading and purity controls for the nuclear and non-nuclear fractions, respectively.