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. 1988 Mar 25;16(5 Pt B):2323–2332. doi: 10.1093/nar/16.5.2323

Hydrogen-bonding effects and 13C-NMR of the DNA double helix.

P N Borer 1, S R LaPlante 1, N Zanatta 1, G C Levy 1
PMCID: PMC338219  PMID: 3357779

Abstract

13C-nmr chemical shifts of the nucleotides in DNA are sensitive to hydrogen bonding, especially for three of the carbons immediately bonded to exocyclic oxygen or nitrogen atoms acting as H-bond acceptors or donors. GuoC2, GuoC6 and ThdC4 are strongly deshielded (about 1 ppm) upon Watson-Crick pairing in oligodeoxynucleotide duplexes, regardless of the base sequence. Deshielding at these sites may be useful to distinguish bases involved in Watson-Crick pairs from unpaired bases.

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Selected References

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