Figure 4.
(A, B, E, F) Indentation stiffness and (C, D, G, H) 45Ca++ uptake by (A, C, E, G) articular cartilage (AC) and (B, D, F, H) growth plate cartilage (GPC) as a function of tissue layer and culture duration up to 3 weeks, n = 6-8 (A-D) and as a function of tissue layer, cell viability, and media supplementation with β-glycerophosphate (βGP) after incubation for 3 weeks, n = 6-8 (E-H). Significant differences from (A-D) t = 1 week or (E-H) from live tissue with 10 mM βGP addition are indicated by a (P < 0.05), b (P < 0.01), and c (P < 0.001).