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. 1988 Jul 25;16(14A):6353–6360. doi: 10.1093/nar/16.14.6353

Detection of an epitope, not required for polymerization, that is conserved between E.coli DNA polymerases I and III and bacteriophage T4 DNA polymerase.

M A Franden 1, C S McHenry 1
PMCID: PMC338300  PMID: 2456526

Abstract

Monoclonal antibodies directed against the alpha subunit of the DNA polymerase III holoenzyme (1) of E. coli were tested for cross-reactivity with a variety of polymerases. We found that one monoclonal antibody bound to E. coli DNA polymerase I as well as to DNA polymerase III. A weaker, but specific, interaction was also detected with T4 DNA polymerase. We exploited the proteolysis procedure developed by Setlow, Brutlag and Kornberg (2) to determine which domain of DNA polymerase I contained the conserved epitope. Contrary to expectations, it was not found in the polymerase domain, but in the 5'----3' exonuclease domain. This reveals a sequence or structure, sufficiently important to be conserved among these polymerases, that is not directly involved in the polymerization reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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