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. 1988 Jul 25;16(14B):6767–6778. doi: 10.1093/nar/16.14.6767

CpG islands and HTF islands in the HLA class I region: investigation of the methylation status of class I genes leads to precise physical mapping of the HLA-B and -C genes.

P Pontarotti 1, G Chimini 1, C Nguyen 1, J Boretto 1, B R Jordan 1
PMCID: PMC338331  PMID: 2841643

Abstract

We have investigated the accessibility of the 5' CpG rich sequences (CpG islands) present in the 5' region of most if not all HLA class I genes to methylation sensitive rare cutter enzymes. We show that for HLA-A, -B, -C genes and a few other (but not all) class I sequences these CpG islands are unmethylated and therefore constitute HTF islands (CpG rich, unmethylated regions of DNA, usually associated with expressed genes). We then map precisely the HTF islands of the HLA-B and HLA-C genes and determine that they are separated by 130 Kb (in agreement with genetic data) and that these two genes are in the same transcriptional orientation on the chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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