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. 1988 Jul 25;16(14B):6789–6802. doi: 10.1093/nar/16.14.6789

IS150: distribution, nucleotide sequence and phylogenetic relationships of a new E. coli insertion element.

E Schwartz 1, M Kröger 1, B Rak 1
PMCID: PMC338333  PMID: 2841644

Abstract

Recently we identified the new insertion (IS) sequence IS150 in various strains of Escherichia coli K-12. We have screened other strains of E. coli and Salmonella typhimurium for the presence of homologous sequences. The strains of E. coli K-12 and W tested contain one or more copies of homology to IS150. We have also determined the complete nucleotide sequence of a copy of IS150 inserted into IS1. Comparison of nucleotide and deduced amino acid sequences of IS150, IS2, IS3, IS51, IS600 and IS629 reveals significant homologies suggesting that these elements are members of a family of phylogenetically related insertion sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Charlier D., Piette J., Glansdorff N. IS3 can function as a mobile promoter in E. coli. Nucleic Acids Res. 1982 Oct 11;10(19):5935–5948. doi: 10.1093/nar/10.19.5935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cullum J., Broda P. Chromosome transfer and Hfr formation by F in rec+ and recA strains of Escherichia coli K12. Plasmid. 1979 Jul;2(3):358–365. doi: 10.1016/0147-619x(79)90019-2. [DOI] [PubMed] [Google Scholar]
  4. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  5. Harley C. B., Reynolds R. P. Analysis of E. coli promoter sequences. Nucleic Acids Res. 1987 Mar 11;15(5):2343–2361. doi: 10.1093/nar/15.5.2343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Iida S., Meyer J., Arber W. Genesis and natural history of IS-mediated transposons. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):27–43. doi: 10.1101/sqb.1981.045.01.006. [DOI] [PubMed] [Google Scholar]
  7. Ishiguro N., Sato G. Nucleotide sequence of insertion sequence IS3411, which flanks the citrate utilization determinant of transposon Tn3411. J Bacteriol. 1988 Apr;170(4):1902–1906. doi: 10.1128/jb.170.4.1902-1906.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jaurin B., Normark S. Insertion of IS2 creates a novel ampC promoter in Escherichia coli. Cell. 1983 Mar;32(3):809–816. doi: 10.1016/0092-8674(83)90067-3. [DOI] [PubMed] [Google Scholar]
  9. Kleckner N. Transposable elements in prokaryotes. Annu Rev Genet. 1981;15:341–404. doi: 10.1146/annurev.ge.15.120181.002013. [DOI] [PubMed] [Google Scholar]
  10. Klock G., Hillen W. Expression, purification and operator binding of the transposon Tn1721-encoded Tet repressor. J Mol Biol. 1986 Jun 20;189(4):633–641. doi: 10.1016/0022-2836(86)90493-6. [DOI] [PubMed] [Google Scholar]
  11. Kröger M., Hobom G. A chain of interlinked genes in the ninR region of bacteriophage lambda. Gene. 1982 Nov;20(1):25–38. doi: 10.1016/0378-1119(82)90084-1. [DOI] [PubMed] [Google Scholar]
  12. Kröger M., Kröger-Block A. Simplified computer programs for search of homology within nucleotide sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):193–201. doi: 10.1093/nar/12.1part1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Louarn J. M., Bouché J. P., Legendre F., Louarn J., Patte J. Characterization and properties of very large inversions of the E. coli chromosome along the origin-to-terminus axis. Mol Gen Genet. 1985;201(3):467–476. doi: 10.1007/BF00331341. [DOI] [PubMed] [Google Scholar]
  14. Matsutani S., Ohtsubo H., Maeda Y., Ohtsubo E. Isolation and characterization of IS elements repeated in the bacterial chromosome. J Mol Biol. 1987 Aug 5;196(3):445–455. doi: 10.1016/0022-2836(87)90023-4. [DOI] [PubMed] [Google Scholar]
  15. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  16. Needleman S. B., Wunsch C. D. A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol. 1970 Mar;48(3):443–453. doi: 10.1016/0022-2836(70)90057-4. [DOI] [PubMed] [Google Scholar]
  17. Ptashne M., Backman K., Humayun M. Z., Jeffrey A., Maurer R., Meyer B., Sauer R. T. Autoregulation and function of a repressor in bacteriophage lambda. Science. 1976 Oct 8;194(4261):156–161. doi: 10.1126/science.959843. [DOI] [PubMed] [Google Scholar]
  18. Rak B., Lusky M., Hable M. Expression of two proteins from overlapping and oppositely oriented genes on transposable DNA insertion element IS5. Nature. 1982 May 13;297(5862):124–128. doi: 10.1038/297124a0. [DOI] [PubMed] [Google Scholar]
  19. Rak B., von Reutern M. Insertion element IS5 contains a third gene. EMBO J. 1984 Apr;3(4):807–811. doi: 10.1002/j.1460-2075.1984.tb01889.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reynolds A. E., Felton J., Wright A. Insertion of DNA activates the cryptic bgl operon in E. coli K12. Nature. 1981 Oct 22;293(5834):625–629. doi: 10.1038/293625a0. [DOI] [PubMed] [Google Scholar]
  21. Ronecker H. J., Rak B. Genetic organization of insertion element IS2 based on a revised nucleotide sequence. Gene. 1987;59(2-3):291–296. doi: 10.1016/0378-1119(87)90337-4. [DOI] [PubMed] [Google Scholar]
  22. Saedler H., Reif H. J., Hu S., Davidson N. IS2, a genetic element for turn-off and turn-on of gene activity in E. coli. Mol Gen Genet. 1974;132(4):265–289. doi: 10.1007/BF00268569. [DOI] [PubMed] [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schnetz K., Toloczyki C., Rak B. Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes. J Bacteriol. 1987 Jun;169(6):2579–2590. doi: 10.1128/jb.169.6.2579-2590.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schwartz E., Herberger C., Rak B. Second-element turn-on of gene expression in an IS1 insertion mutant. Mol Gen Genet. 1988 Feb;211(2):282–289. doi: 10.1007/BF00330605. [DOI] [PubMed] [Google Scholar]
  26. Sengstag C., Arber W. IS2 insertion is a major cause of spontaneous mutagenesis of the bacteriophage P1: non-random distribution of target sites. EMBO J. 1983;2(1):67–71. doi: 10.1002/j.1460-2075.1983.tb01382.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shepherd J. C. Method to determine the reading frame of a protein from the purine/pyrimidine genome sequence and its possible evolutionary justification. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1596–1600. doi: 10.1073/pnas.78.3.1596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Smith G. E., Summers M. D. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Anal Biochem. 1980 Nov 15;109(1):123–129. doi: 10.1016/0003-2697(80)90019-6. [DOI] [PubMed] [Google Scholar]
  30. Timmerman K. P., Tu C. P. Complete sequence of IS3. Nucleic Acids Res. 1985 Mar 25;13(6):2127–2139. doi: 10.1093/nar/13.6.2127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Yamada T., Lee P. D., Kosuge T. Insertion sequence elements of Pseudomonas savastanoi: Nucleotide sequence and homology with Agrobacterium tumefaciens transfer DNA. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8263–8267. doi: 10.1073/pnas.83.21.8263. [DOI] [PMC free article] [PubMed] [Google Scholar]

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