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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Laryngoscope. 2011 Nov 22;122(1):174–189. doi: 10.1002/lary.22392

Figure 5. AR42 exposure decreased p-AKT staining and Ki-67 labeling in treated human VS xenografts.

Figure 5

VS tumors from the mice described in Figure 4 were excised after the final MRI imaging sequence, fixed in formalin, and paraffin embedded. Immunostaining performed for phospho-Ser473-AKT revealed an ~ 50% decline in p-AKT staining for AR42-treated tumors versus control sections. Likewise, Ki-67 labeling revealed that AR42 treatment caused an ~ 50% decrease in the proliferative index. Staining sections with cleaved caspase-3 antibody was performed analysis to evaluate apoptosis. AR42-treated tumors had modestly more cleaved caspase-3 positivity compared to control tumors, but the labeling was sparse.