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. 1988 Sep 12;16(17):8487–8496. doi: 10.1093/nar/16.17.8487

Intrinsic polymorphism of variable number tandem repeat loci in the human genome.

S Ali 1, R B Wallace 1
PMCID: PMC338571  PMID: 2901724

Abstract

In the human genome, short tandem repetitive (STR) DNA sequences often show restriction fragment length polymorphisms (RFLPs) due to variation in the number of copies of the repeat unit. For a subset of these sequences known as minisatellites or variable number tandem repeat loci (VNTR), it has been proposed that a homologous "core" sequence of 10-12 nucleotides is involved in the mechanism(s) generating the polymorphism. In our present study we have prepared oligonucleotide probes complementary to one or two repeat units of several VNTR loci. Under stringent hybridization and wash conditions these probes hybridize locus specifically thus allowing the evaluation of the intrinsic polymorphism of individual loci. Our results indicate that not all of the loci having STR DNA sequences are polymorphic despite the fact that they share the "core" sequence. This suggests that more than the DNA sequence of the locus is involved in the mechanism(s) generating the polymorphism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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