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. 1988 Sep 26;16(18):8835–8846. doi: 10.1093/nar/16.18.8835

5' flanking sequence and genomic structure of Egr-1, a murine mitogen inducible zinc finger encoding gene.

C H Tsai-Morris 1, X M Cao 1, V P Sukhatme 1
PMCID: PMC338638  PMID: 3140220

Abstract

Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and consists of 2 exons and one 700 bp intron. S1 nuclease protection and primer extension analysis were used to define the transcription initiation site. "TATA" and "CCAAT" sequences were located at nucleotides -26 and -337 respectively. In addition, there exist five elements whose sequence is nearly identical to the inner core 10 nucleotide region (CCATATTAGG) of the c-fos serum response element, four Sp1 consensus sequences, two AP1 target sequence analogs, and two potential cAMP response elements. These results will ultimately lead to a detailed definition of the intracellular events regulating Egr-1 expression.

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Selected References

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