Fig. 7.

The effect of various waters with or without alloxan on body weight (a), and food (b) and water (c) consumption. a Body weight was measured at the time of experimental initiation (designated as 0 weeks), and at the 4th and 8th weeks. Body weight was compared between alloxan-treated and non-treated mice. Values are means ± standard error of the mean calculated from the data of mice that survived. *p < 0.05, UPW(+)ALX compared with Hita T. W.(+)ALX or Nord. W.(+)ALX. For food and water consumption measurements, one group was placed in three cages in which three mice were placed in each cage. Consumed food and water were measured as the total weight per cage. The time course of food (b) consumption in normal and alloxan-injected mice was measured at the indicated time points (0–9 weeks). Food consumption is expressed as means ± standard error of the mean (n = 3). ⁺⁺p < 0.01, ⁺⁺⁺p < 0.001, UPW(+)ALX compared with various waters with alloxan injection. ^#p < 0.05, Nord. W.(+)ALX compared with CNMW(+)ALX. ^$p < 0.05, Nord. W.(+)ALX compared with UPW(+)ALX. Group designations are as follows: open circle ultrapure water (UPW) without alloxan; closed circle UPW plus 1 mM alloxan; open square a brand of commercialized natural mineral water plus 1 mM alloxan; open diamond Nordenau water plus 1 mM alloxan; closed square Hita Tenryosui water plus 1 mM alloxan. The time course of water (c) consumption in normal and alloxan-injected mice was measured at the indicated time points. Water consumption is expressed as means ± standard error of the mean (n = 3). ⁺⁺p < 0.01, ⁺⁺⁺p < 0.001, UPW(ALX) compared with various waters with alloxan injection. ^#p < 0.05, ^##p < 0.01, CNMW(+)ALX compared with Nord. W.(+)ALX or Hita T. W.(+)ALX. Group designations are the same as those in b