Table 2.
Real-time PCR amplifications of cDNA isolated from FIV-infected CD4+ T-cells.
| Cat Number | Gag Copies | R Copies | R-U5 Copies | OrfA Copies |
|---|---|---|---|---|
| 165 | BLD | 16,000 (±2,000) | BLD | BLD |
| 184 | BLD | 30,000 (±20,000) | BLD | BLD |
| 186 | BLD | 3,900 (±900) | BLD | BLD |
| 187 | BLD | 20,000 (±10,000) | BLD | BLD |
| 184(A) | 1,600 (±100) | 690,000 (±60,000) | 1,200 (±100) | 3,100 (±300) |
Data are presented as the mean (± standard deviation) of triplicate PCR measurements per 106 copies feline GAPDH cDNA, and are representative of CD4+ T-cell isolation at two different time points (30–34 months post FIV infection) for each cat. Sample labeled 184(A) represents PBMC from cat 184 during a period of viral activation in blood monocytes; BLD indicates values below the limit of detection.