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. 1988 Oct 11;16(19):9165–9176. doi: 10.1093/nar/16.19.9165

Chemical synthesis of DNA oligomers containing cytosine arabinoside.

G P Beardsley 1, T Mikita 1, M M Klaus 1, A L Nussbaum 1
PMCID: PMC338698  PMID: 3174449

Abstract

The solid phase phospite triester synthesis of oligodeoxynucleotides containing cytosine arabinoside (araC) is described. A protected araC phosphoramadite was prepared for the introduction of araC residues at 5'termini and internucleotide positions in DNA oligomers. These oligomers were utilized to demonstrate the formation of correct 3'-5' linkages, to test for alkaline lability at the araC site, and to study the stability of duplexes containing araC-G base pairs. For the introduction of araC residues at 3' terminal positions, a protected derivative of araC was coupled to functionalized silica. This material was used to prepare a test oligomer which was characterized enzymatically.

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Selected References

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