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The interaction energy between the A3 helix and the P-domain helices P5,P6,P7 and P8 during the E1P -> E2P transition. The x-axis shows the progress of the transition from the starting state (2ZBD) to the ending state (1WPG). The transitions are divided into 5% percent completion bins and the values are averaged over 50 transitions for each mutant. Error bars represent the standard deviation within each bin.
