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. 1988 Nov 11;16(21):10109–10118. doi: 10.1093/nar/16.21.10109

Turnover products of the apo very low density lipoprotein II messenger RNA from chicken liver.

O Bakker 1, A C Arnberg 1, M H Noteborn 1, A J Winter 1, G Ab 1
PMCID: PMC338840  PMID: 3194196

Abstract

The mature apo Very Low Density Lipoprotein II (apo VLDLII) mRNA appears in chicken liver within a few hours after estrogen administration. Apart from this mRNA species, shorter RNA molecules hybridizing to apo VLDLII sequences have been detected in rooster liver upon estrogen stimulation. These molecules are present in the non-polyadenylated fraction of the total cellular- and polysomal RNA. Northern blotting and electron microscopy of R-loops were employed to show that these shorter RNA molecules are truncated at their 3'-end. The 3'-termini were further characterized by nuclease S1 analyses, and are located predominantly in the 3' untranslated region of the mRNA. Using a secondary structure model (Shelness and Williams, J. Biol. Chem. 260, 8637-8646, 1985), we show that the 3' termini map mainly in unpaired regions of the structure.

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Selected References

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