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. Author manuscript; available in PMC: 2012 Jul 3.
Published in final edited form as: Gastroenterology. 2009 Nov 13;138(3):958–968. doi: 10.1053/j.gastro.2009.11.005

Figure 1.

Figure 1

Cell culture and mouse model: murine model of metastasis, in vivo monitoring, and ex vivo proof of metastases. (A) MC-38 parental cells (heterogeneous, blue and red) were subjected to 6 sequential passages through matrigel-coated transwells (enrichment of invasive subpopulations of MC-38 cells [red]) called “MC-38inv.” After in vivo passage, a stabilized cell line (pink cells) called “MC-38met” was established. (B) MC-38inv cells were tested alongside MC-38 parental cells for the ability to form lung metastasis in a tail vein assay. The figure shows representative tumor progression in live mice by bioluminescent imaging (days 1–21) and at the time of autopsy (day 21).