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. 1988 Nov 25;16(22):10833–10847. doi: 10.1093/nar/16.22.10833

A related moderately repetitive DNA family in the nematodes Ascaris lumbricoides and Panagrellus silusiae.

T Warren 1, J J Pasternak 1
PMCID: PMC338942  PMID: 3205722

Abstract

Digestion of genomic DNA from the nematodes Panagrellus silusiae and Ascaris lumbricoides with restriction endonuclease BamH1 releases a 0.7 kilobase (kb) fragment. The 0.7 kb fragment from both nematodes was cloned onto E. coli plasmid pUC19. Using representative clones as DNA hybridization probes, it was found that (i) the BamH1 fragments cross-hybridize; (ii) a ladder-effect with multiples of 0.7 kb was evident in both species after hybridization to genomic DNA and (iii) the genomic copy number of BamH1 elements is 150 and 195 for P. silusiae and A. lumbricoides respectively. DNA sequence analysis of the inserts, AL700-1 and PS700-1, revealed nucleotide blocks with over 85% similarity. No open reading frames are present in either DNA fragment. Neither fragment hybridizes to genomic DNA from Caenorhabditis elegans. Northern blot hybridization indicated that the 0.7 kb element is transcribed into poly(A)(-)-RNA in P. silusiae; but, is not transcribed in adult Ascaris muscle. Thus, P. silusiae and A. lumbricoides share a homologous, tandemly arrayed, moderately repetitive DNA family.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aeby P., Spicher A., de Chastonay Y., Müller F., Tobler H. Structure and genomic organization of proretrovirus-like elements partially eliminated from the somatic genome of Ascaris lumbricoides. EMBO J. 1986 Dec 1;5(12):3353–3360. doi: 10.1002/j.1460-2075.1986.tb04650.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Back E., Van Meir E., Müller F., Schaller D., Neuhaus H., Aeby P., Tobler H. Intervening sequences in the ribosomal RNA genes of Ascaris lumbricoides: DNA sequences at junctions and genomic organization. EMBO J. 1984 Nov;3(11):2523–2529. doi: 10.1002/j.1460-2075.1984.tb02167.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beauchamp R. S., Pasternak J., Straus N. A. Characterization of the genome of the free-living nematode Panagrellus silusiae: absence of short period interspersion. Biochemistry. 1979 Jan 23;18(2):245–251. doi: 10.1021/bi00569a001. [DOI] [PubMed] [Google Scholar]
  4. Behme R., Pasternak J. DNA base composition of some free-living nematode species. Can J Genet Cytol. 1969 Dec;11(4):993–1000. doi: 10.1139/g69-116. [DOI] [PubMed] [Google Scholar]
  5. Bouchard R. A. Moderately repetitive DNA in evolution. Int Rev Cytol. 1982;76:113–193. doi: 10.1016/s0074-7696(08)61790-8. [DOI] [PubMed] [Google Scholar]
  6. Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974 May;77(1):71–94. doi: 10.1093/genetics/77.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chen E. Y., Seeburg P. H. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA. 1985 Apr;4(2):165–170. doi: 10.1089/dna.1985.4.165. [DOI] [PubMed] [Google Scholar]
  8. Davidson E. H., Galau G. A., Angerer R. C., Britten R. J. Comparative aspects of DNA organization in Metazoa. Chromosoma. 1975 Jul 21;51(3):253–259. doi: 10.1007/BF00284818. [DOI] [PubMed] [Google Scholar]
  9. Dowsett A. P. Closely related species of Drosophila can contain different libraries of middle repetitive DNA sequences. Chromosoma. 1983;88(2):104–108. doi: 10.1007/BF00327329. [DOI] [PubMed] [Google Scholar]
  10. Hwu H. R., Roberts J. W., Davidson E. H., Britten R. J. Insertion and/or deletion of many repeated DNA sequences in human and higher ape evolution. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3875–3879. doi: 10.1073/pnas.83.11.3875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacobson J. W., Medhora M. M., Hartl D. L. Molecular structure of a somatically unstable transposable element in Drosophila. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8684–8688. doi: 10.1073/pnas.83.22.8684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kumazaki T., Hori H., Osawa S. Phylogeny of protozoa deduced from 5S rRNA sequences. J Mol Evol. 1983;19(6):411–419. doi: 10.1007/BF02102316. [DOI] [PubMed] [Google Scholar]
  13. Link C. D., Graf-Whitsel J., Wood W. B. Isolation and characterization of a nematode transposable element from Panagrellus redivivus. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5325–5329. doi: 10.1073/pnas.84.15.5325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lohe A. R., Brutlag D. L. Identical satellite DNA sequences in sibling species of Drosophila. J Mol Biol. 1987 Mar 20;194(2):161–170. doi: 10.1016/0022-2836(87)90365-2. [DOI] [PubMed] [Google Scholar]
  15. Marck C. Fast analysis of DNA and protein sequence on Apple IIe: restriction sites search, alignment of short sequence and dot matrix analysis. Nucleic Acids Res. 1986 Jan 10;14(1):583–590. doi: 10.1093/nar/14.1.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McReynolds L. A., DeSimone S. M., Williams S. A. Cloning and comparison of repeated DNA sequences from the human filarial parasite Brugia malayi and the animal parasite Brugia pahangi. Proc Natl Acad Sci U S A. 1986 Feb;83(3):797–801. doi: 10.1073/pnas.83.3.797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  18. Moore G. P., Pearson W. R., Davidson E. H., Britten R. J. Long and short repeats of sea urchin DNA and their evolution. Chromosoma. 1981;84(1):19–32. doi: 10.1007/BF00293360. [DOI] [PubMed] [Google Scholar]
  19. Müller F., Walker P., Aeby P., Neuhaus H., Felder H., Back E., Tobler H. Nucleotide sequence of satellite DNA contained in the eliminated genome of Ascaris lumbricoides. Nucleic Acids Res. 1982 Dec 11;10(23):7493–7510. doi: 10.1093/nar/10.23.7493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perler F. B., Karam M. Cloning and characterization of two Onchocerca volvulus repeated DNA sequences. Mol Biochem Parasitol. 1986 Nov;21(2):171–178. doi: 10.1016/0166-6851(86)90020-4. [DOI] [PubMed] [Google Scholar]
  21. Rosenzweig B., Liao L. W., Hirsh D. Sequence of the C. elegans transposable element Tc1. Nucleic Acids Res. 1983 Jun 25;11(12):4201–4209. doi: 10.1093/nar/11.12.4201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Roth G. E., Moritz K. B. Restriction enzyme analysis of the germ line limited DNA of Ascaris suum. Chromosoma. 1981;83(2):169–190. doi: 10.1007/BF00286787. [DOI] [PubMed] [Google Scholar]
  23. Rothstein M. Practical methods for the axenic culture of the free-living nematodes Turbatrix aceti and Caenorhabditis briggsae. Comp Biochem Physiol B. 1974 Dec 15;49(4):669–678. doi: 10.1016/0305-0491(74)90254-5. [DOI] [PubMed] [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  26. Staden R. Sequence data handling by computer. Nucleic Acids Res. 1977 Nov;4(11):4037–4051. doi: 10.1093/nar/4.11.4037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sternberg P. W., Horvitz H. R. Gonadal cell lineages of the nematode Panagrellus redivivus and implications for evolution by the modification of cell lineage. Dev Biol. 1981 Nov;88(1):147–166. doi: 10.1016/0012-1606(81)90226-8. [DOI] [PubMed] [Google Scholar]
  28. Sternberg P. W., Horvitz H. R. Postembryonic nongonadal cell lineages of the nematode Panagrellus redivivus: description and comparison with those of Caenorhabditis elegans. Dev Biol. 1982 Sep;93(1):181–205. doi: 10.1016/0012-1606(82)90251-2. [DOI] [PubMed] [Google Scholar]
  29. Streeck R. E., Moritz K. B., Beer K. Chromatin diminution in Ascaris suum: nucleotide sequence of the eliminated satellite DNA. Nucleic Acids Res. 1982 Jun 11;10(11):3495–3502. doi: 10.1093/nar/10.11.3495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sulston J. E., Brenner S. The DNA of Caenorhabditis elegans. Genetics. 1974 May;77(1):95–104. doi: 10.1093/genetics/77.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Thomas P. S. Hybridization of denatured RNA transferred or dotted nitrocellulose paper. Methods Enzymol. 1983;100:255–266. doi: 10.1016/0076-6879(83)00060-9. [DOI] [PubMed] [Google Scholar]
  32. Weiner A. M., Deininger P. L., Efstratiadis A. Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information. Annu Rev Biochem. 1986;55:631–661. doi: 10.1146/annurev.bi.55.070186.003215. [DOI] [PubMed] [Google Scholar]
  33. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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