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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Prostate. 2012 Mar 27;72(15):1628–1637. doi: 10.1002/pros.22516

Figure 2. Changes in tumor mRNA expression of classical calcitriol target genes.

Figure 2

PC-3 xenografts were harvested from mice at the end of four weeks of treatment. Total RNA was isolated from harvested tumor tissue and mRNA levels of the calcitriol inactivating enzyme CYP24A1 (A), anti-proliferative genes p21 and IGFBP-3 (A), apoptosis-related genes Bcl-2 and Bax (B), and cell cycle promoter cyclin D1 (B) were analyzed by qRT-PCR, as described in Materials and Methods. Relative mRNA expression for each gene in tumors from control mice was set at 1. Values represent mean ± SE of 6–11 measurements in each group. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to control. + p < 0.05, ++ p< 0.01 compared to calcitriol. ^ p< 0.05, ^^ p < 0.01 compared to 20%-soy.