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. 1988 Dec 23;16(24):11725–11735. doi: 10.1093/nar/16.24.11725

Characterization of RNA hairpin loop stability.

D R Groebe 1, O C Uhlenbeck 1
PMCID: PMC339106  PMID: 3211748

Abstract

Fifteen RNA hairpins that share the same stem sequence and have homopolymer loops of A, C and U residues which vary in length from three to nine nucleotides were synthesized and their thermal stabilities determined. Tm varies as a function of loop size but is almost independent of loop composition. Loops of four or five nucleotides are found to be the most stable loop size. This is consistent with the observation that four-membered loops are the most prevalent loop size in 16S-like RNAs. The contribution of each loop to hairpin stability was calculated by subtracting the known contribution of the helical stem. These data should be useful for predicting the stability of other hairpins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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