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Secreted proteins from BMVEC cultured media after a 48 hr treatment with EtOH or 2 hr with AA were analyzed for levels of (A) TIMP-1 or (B) TIMP-2 expression using the ELISA kits. TIMPs levels were calculated from standard curve and results were expressed as ng/mg protein (± SEM; n = 4). Respective inhibitor did not change the TIMPs expression of basal controls.
