Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1986 Jan 10;14(1):575–581. doi: 10.1093/nar/14.1.575

SPLINT: a cubic spline interpolation program for the analysis of fragment sizes in one-dimensional electrophoresis gels.

C E Gariepy, M I Lomax, L I Grossman
PMCID: PMC339444  PMID: 3003683

Abstract

SPLINT (SPLine INTerpolation) is a BASIC microcomputer program that uses electrophoretic data on the migration distances of DNA fragments of known size to approximate the relationship between distance moved and DNA fragment size by a piecewise cubic spline function. This function places all standards exactly on the calibration curve and then determines from it the sizes of other fragments in the gel. Special advantages of this program include the use of a digitizer to enter the fragment positions directly from a gel photograph and the optional generation of a graph of the approximating function plus the placement on it of points from any specified gel lanes.

Full text

PDF
575

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gough E. J., Gough N. M. Direct calculation of the sizes of DNA fragments separated by gel electrophoresis using programmes written for a pocket calculator. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):845–853. doi: 10.1093/nar/12.1part2.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Grabowski P. J., Padgett R. A., Sharp P. A. Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate. Cell. 1984 Jun;37(2):415–427. doi: 10.1016/0092-8674(84)90372-6. [DOI] [PubMed] [Google Scholar]
  3. Gray A. J., Beecher D. E., Olson M. V. Computer-based image analysis of one-dimensional electrophoretic gels used for the separation of DNA restriction fragments. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):473–491. doi: 10.1093/nar/12.1part2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kieser T. DNAGEL: a computer program for determining DNA fragment sizes using a small computer equipped with a graphics tablet. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):679–688. doi: 10.1093/nar/12.1part2.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Loucks E., Chaconas G., Blakesley R. W., Wells R. D., van de Sande J. H. Antibiotic induced electrophoretic mobility shifts of DNA restriction fragments. Nucleic Acids Res. 1979;6(5):1869–1879. doi: 10.1093/nar/6.5.1869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Maina C. V., Nolan G. P., Szalay A. A. Molecular weight determination program. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):695–702. doi: 10.1093/nar/12.1part2.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Marini J. C., Levene S. D., Crothers D. M., Englund P. T. Bent helical structure in kinetoplast DNA. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7664–7668. doi: 10.1073/pnas.79.24.7664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moore K. H., Johnson P. H., Chandler S. E., Grossman L. I. A restriction endonuclease cleavage map of mouse mitochondrial DNA. Nucleic Acids Res. 1977;4(5):1273–1289. doi: 10.1093/nar/4.5.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rood J. I., Gawthorne J. M. Apple software for analysis of the size of restriction fragments. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):689–694. doi: 10.1093/nar/12.1part2.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Russell P. J., Crandall R. E., Feinbaum R. GELYSIS: Pascal-implemented analysis of one-dimensional electrophoresis gels. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):493–498. doi: 10.1093/nar/12.1part2.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sanger F., Coulson A. R., Hong G. F., Hill D. F., Petersen G. B. Nucleotide sequence of bacteriophage lambda DNA. J Mol Biol. 1982 Dec 25;162(4):729–773. doi: 10.1016/0022-2836(82)90546-0. [DOI] [PubMed] [Google Scholar]
  13. Schaffer H. E., Sederoff R. R. Improved estimation of DNA fragment lengths from Agarose gels. Anal Biochem. 1981 Jul 15;115(1):113–122. doi: 10.1016/0003-2697(81)90533-9. [DOI] [PubMed] [Google Scholar]
  14. Southern E. Gel electrophoresis of restriction fragments. Methods Enzymol. 1979;68:152–176. doi: 10.1016/0076-6879(79)68011-4. [DOI] [PubMed] [Google Scholar]
  15. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  16. Wu H. M., Crothers D. M. The locus of sequence-directed and protein-induced DNA bending. Nature. 1984 Apr 5;308(5959):509–513. doi: 10.1038/308509a0. [DOI] [PubMed] [Google Scholar]
  17. Zeiger R. S., Salomon R., Dingman C. W., Peacock A. C. Role of base composition in the electrophoresis of microbial and crab DNA in polyacrylamide gels. Nat New Biol. 1972 Jul 19;238(81):65–69. doi: 10.1038/newbio238065a0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES