Abstract
Totipotent leaf mesophyll protoplasts of Nicotiana plumbaginifolia, Viviani were inoculated with cassava latent virus (CLV) or with full length copies of CLV genomic DNAs 1 and 2 excised from replicative forms of M13 clones. Virus specific DNAs began to appear 48-72h after inoculation with virus or cloned DNAs, coincident with the onset of host cell division. Infected cells accumulated supercoiled forms of DNAs 1 and 2 as well as progeny single-stranded (ss) virion (+) sense DNAs representing each component of the genome. Both supercoiled and ss molecules were synthesised by cells inoculated with cloned DNA 1 alone but DNA 2 failed to replicate independently.
Full text
PDF












Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
- Gay N. J., Walker J. E. Homology between human bladder carcinoma oncogene product and mitochondrial ATP-synthase. Nature. 1983 Jan 20;301(5897):262–264. doi: 10.1038/301262a0. [DOI] [PubMed] [Google Scholar]
- Hamilton W. D., Bisaro D. M., Buck K. W. Identification of novel DNA forms in tomato golden mosaic virus infected tissue. Evidence for a two component viral genome. Nucleic Acids Res. 1982 Aug 25;10(16):4901–4912. doi: 10.1093/nar/10.16.4901. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hull R., Davies J. W. Genetic engineering with plant viruses, and their potential as vectors. Adv Virus Res. 1983;28:1–33. doi: 10.1016/s0065-3527(08)60720-4. [DOI] [PubMed] [Google Scholar]
- Ikegami M., Haber S., Goodman R. M. Isolation and characterization of virus-specific double-stranded DNA from tissues infected by bean golden mosaic virus. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4102–4106. doi: 10.1073/pnas.78.7.4102. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koths K., Dressler D. Analysis of the phiX DNA replication cycle by electron microscopy. Proc Natl Acad Sci U S A. 1978 Feb;75(2):605–609. doi: 10.1073/pnas.75.2.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pritchard C., Patton J. T., Bates R. C., Stout E. R. Replication of nondefective parvoviruses: lack of a virion-associated DNA polymerase. J Virol. 1978 Oct;28(1):20–27. doi: 10.1128/jvi.28.1.20-27.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Stanley J. The molecular biology of geminiviruses. Adv Virus Res. 1985;30:139–177. doi: 10.1016/s0065-3527(08)60450-9. [DOI] [PubMed] [Google Scholar]
- Stanley J., Townsend R. Characterisation of DNA forms associated with cassava latent virus infection. Nucleic Acids Res. 1985 Apr 11;13(7):2189–2206. doi: 10.1093/nar/13.7.2189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tennant R. W., Hand R. E., Jr Requirement of cellular synthesis for Kilham rat virus replication. Virology. 1970 Dec;42(4):1054–1063. doi: 10.1016/0042-6822(70)90353-3. [DOI] [PubMed] [Google Scholar]
- Townsend R., Stanley J., Curson S. J., Short M. N. Major polyadenylated transcripts of cassava latent virus and location of the gene encoding coat protein. EMBO J. 1985 Jan;4(1):33–37. doi: 10.1002/j.1460-2075.1985.tb02313.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]