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. 1986 Feb 11;14(3):1379–1391. doi: 10.1093/nar/14.3.1379

Nucleotide sequence of the chicken 5-aminolevulinate synthase gene.

D J Maguire, A R Day, I A Borthwick, G Srivastava, P L Wigley, B K May, W H Elliott
PMCID: PMC339511  PMID: 3005973

Abstract

5-Aminolevulinate synthase, the first and rate-controlling enzyme of heme biosynthesis, is regulated in the liver by the end-product heme. To study this negative control mechanism, we have isolated the chicken gene for ALA-synthase and determined the nucleotide sequence. The structural gene is 6.9 kb long and contains 10 exons. The transcriptional start site for ALA-synthase was determined by primer extension analysis. A fragment of 291 bp from the 5' flanking region including 34 bp of the first exon shows promoter activity when introduced upstream of a chicken histone H2B gene and injected into the nuclei of Xenopus laevis oocytes.

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Selected References

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