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. 1986 Feb 25;14(4):1565–1581. doi: 10.1093/nar/14.4.1565

Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus.

M Zalacain, A González, M C Guerrero, R J Mattaliano, F Malpartida, A Jiménez
PMCID: PMC339529  PMID: 3005976

Abstract

The nucleotide sequence of a 1467 bp fragment of Streptomyces hygroscopicus DNA containing the gene (hyg) encoding a hygromycin B phosphotransferase (HPH) has been determined. The N-terminal amino acid sequence of HPH determined by automated Edman degradation has allowed the coding sequence of the hyg gene to be identified. The translation initiation triplet is GTG and 5 bp preceding it there is a sequence complementary to the 3'-end of 16S rRNA from S. lividans. The transcriptional start and termination sites have been determined; the presumptive promoter region has only partial homology to that of the Streptomyces vinaceus vph gene and is different to the promoter sequences of other Streptomyces genes.

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Selected References

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