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. 1986 Feb 25;14(4):1719–1726. doi: 10.1093/nar/14.4.1719

Differential effects of dimethylsulfoxide on the activities of human DNA polymerases alpha and delta.

M Y Lee, N L Toomey
PMCID: PMC339568  PMID: 3951994

Abstract

The effects of dimethylsulfoxide on the activities of purified human placental DNA polymerase alpha and DNA polymerase delta were examined. DNA polymerase alpha was inhibited by dimethylsulfoxide, whereas DNA polymerase delta was significantly activated, by as much as 6-fold. Kinetic data show that the effect of dimethylsulfoxide on DNA polymerase delta activity was due to a reduction in the apparent Km for its substrate, dTTP. This novel finding of the differential effects of dimethylsulfoxide on the activities of polymerases alpha and delta may be useful in their identification and differential assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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