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. 1986 Mar 11;14(5):2001–2014. doi: 10.1093/nar/14.5.2001

Footprinting reveals that nogalamycin and actinomycin shuffle between DNA binding sites.

K R Fox, M J Waring
PMCID: PMC339639  PMID: 2421246

Abstract

The hypothesis that sequence-selective DNA-binding antibiotics locate their preferred binding sites by a process involving migration from nonspecific sites has been tested by footprinting with DNAase I. Footprinting patterns on the tyrT DNA fragment produced by nogalamycin and actinomycin change with time after mixing the antibiotic with the DNA. Sites of protection as well as enhanced cleavage are seen to develop in a fashion which is both temperature and concentration-dependent. At certain sites cutting is transiently enhanced, then blocked. Limited evidence for slow reaction with echinomycin and mithramycin is presented, but the kinetics of footprinting with daunomycin and distamycin appear instantaneous. The feasibility of adducing direct evidence for shuffling by footprinting seems to be governed by slow dissociation of the antibiotic-DNA complex. It may also be dependent upon the mode of binding, be it intercalative or non-intercalative in character.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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