CELL BIOLOGY Correction for “mTOR associates with TFIIIC, is found at tRNA and 5S rRNA genes, and targets their repressor Maf1,” by Theodoros Kantidakis, Ben A. Ramsbottom, Joanna L. Birch, Sarah N. Dowding, and Robert J. White, which appeared in issue 26, June 29, 2010, of Proc Natl Acad Sci USA (107:11823–11828; first published June 11, 2010; 10.1073/pnas.1005188107).
The authors note that Fig. 4 appeared incorrectly. The corrected figure and its legend appear below. These errors do not affect the conclusions of the article.
Fig. 4.
TFIIIC associates with mTOR. (A) Western blot analysis using mTOR and raptor antibodies of input and immunoprecipitates produced with nonimmune IgG or antibody to TFIIIC110. (B) Western blot analysis using TFIIIC110 antibody of input and immunoprecipitates produced with nonimmune IgG and antibodies to raptor and mTOR. (C) Western blot analysis using TFIIIC110 antibody of immunoprecipitates produced in the presence (+) or absence (−) of DNase I with nonimmune IgG and antibodies to raptor and mTOR. (D) PLA using antibodies against mTOR and TFIIIC220. (E) PLA using antibodies against mTOR and TFIIIC110. (F) PLA using antibodies against mTOR and UBF. (G) PLA using two antibodies against UBF. (H) PLA using antibodies against mTOR and Maf1. (I) Quantification of PLA assays conducted using antibodies against the indicated pairs of proteins or without primary antibodies. The well-characterized interaction between TFIIIB subunits TBP and Brf1 was included as a positive control. GFP provides a negative control, because it is not expressed in these cells.