Figure 5. Intracellular survival assay of ΔSTM1485 in mouse macrophage cells (A) and complementation studies in INT-407 (B) and RAW264.7 (C). Cells were infected with WT or ΔSTM1485 or ΔSTM1485pQE601485 strains were lysed at 2 and 16 h post infection. Bacterial load was shown as fold increase in CFU from 2 h to 16 h. Graphs are representative of two independent experiments with similar results. Statistical significance was defined as follows (**p < 0.005) (Student's t-test).