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. 1986 Mar 25;14(6):2567–2582. doi: 10.1093/nar/14.6.2567

Protein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease.

A T Yeung, W B Mattes, L Grossman
PMCID: PMC339683  PMID: 3960727

Abstract

An examination has been made into the nature of the nucleoprotein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease when acting on a pyrimidine dimer-containing fd RF-I DNA species. The complexes of proteins and DNA form in unique stages. The first stage of binding involves an ATP-stimulated interaction of the UvrA protein with duplex DNA containing pyrimidine dimer sites. The UvrB protein significantly stabilizes the UvrA-pyrimidine dimer containing DNA complex which, in turn, provides a foundation for the binding of UvrC to activate the UvrABC endonuclease. The binding of one molecule of UvrC to each UvrAB-damaged DNA complex is needed to catalyze incision in the vicinity of pyrimidine dimer sites. The UvrABC-DNA complex persists after the incision event suggesting that the lack of UvrABC turnover may be linked to other activities in the excision-repair pathway beyond the initial incision reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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