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. 1986 Apr 25;14(8):3197–3214. doi: 10.1093/nar/14.8.3197

Nucleotide sequence of an heterochromatic segment recognized by the antibodies to Z-DNA in fixed metaphase chromosomes.

B Malfoy, N Rousseau, N Vogt, E Viegas-Pequignot, B Dutrillaux, M Leng
PMCID: PMC339742  PMID: 3010230

Abstract

The purpose of this work was to analyse at the molecular level the DNA recognized by the antibodies to Z-DNA in in situ experiments. Antibodies to Z-DNA interact strongly with R-band positive heterochromatic segments of fixed metaphase chromosomes of Cebus (Viegas-Pequignot et al., 1983). These segments are constituted of a satellite DNA the repeat unit of which is about 1520 base pairs long. The base sequence of the repeat unit has been determined. It contains a (AC)n rich region which, in vitro, adopts the Z conformation under topological constraints. Experiments with nuclei suggest that this sequence is not predominantly in the Z conformation in vivo. The polymorphic structure of the (AC)n rich region argues for an active recombination sequence.

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  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Camerini-Otero R. D., Felsenfeld G. Supercoiling energy and nucleosome formation: the role of the arginine-rich histone kernel. Nucleic Acids Res. 1977;4(5):1159–1181. doi: 10.1093/nar/4.5.1159-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Couturier J., Cuny G., Hudson A. P., Dutrillaux B., Bernardi G. Cytogenetical and biochemical characterization of a dG + dC-rich satellite DNA in the primate Cebus capucinus. Biochimie. 1982 Jun;64(6):443–450. doi: 10.1016/s0300-9084(82)80583-x. [DOI] [PubMed] [Google Scholar]
  4. Goodwin G. H., Nicolas R. H., Cockerill P. N., Zavou S., Wright C. A. The effect of salt extraction on the structure of transcriptionally active genes; evidence for a DNAseI-sensitive structure which could be dependent on chromatin structure at levels higher than the 30 nm fibre. Nucleic Acids Res. 1985 May 24;13(10):3561–3579. doi: 10.1093/nar/13.10.3561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gross D. S., Huang S. Y., Garrard W. T. Chromatin structure of the potential Z-forming sequence (dT-dG)n X (dC-dA)n. Evidence for an "alternating-B" conformation. J Mol Biol. 1985 May 25;183(2):251–265. doi: 10.1016/0022-2836(85)90218-9. [DOI] [PubMed] [Google Scholar]
  6. Guigues M., Leng M. Reactivity of antibodies to guanosine modified by the carcinogen N-acetoxy-N-2-acetylaminofluorene. Nucleic Acids Res. 1979 Feb;6(2):733–744. doi: 10.1093/nar/6.2.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  8. Heaphy S., Tregear R. Stoichiometry of covalent actin-subfragment 1 complexes formed on reaction with a zero-length cross-linking compound. Biochemistry. 1984 May 8;23(10):2211–2214. doi: 10.1021/bi00305a017. [DOI] [PubMed] [Google Scholar]
  9. Hill R. J., Watt F., Stollar B. D. Z-DNA immunoreactivity of Drosophila polytene chromosomes. Effects of the fixatives 45% acetic acid and 95% ethanol and of DNase I nicking. Exp Cell Res. 1984 Aug;153(2):469–482. doi: 10.1016/0014-4827(84)90614-1. [DOI] [PubMed] [Google Scholar]
  10. Hsieh T. S., Wang J. C. Thermodynamic properties of superhelical DNAs. Biochemistry. 1975 Feb 11;14(3):527–535. doi: 10.1021/bi00674a011. [DOI] [PubMed] [Google Scholar]
  11. Kmiec E. B., Angelides K. J., Holloman W. K. Left-handed DNA and the synaptic pairing reaction promoted by Ustilago rec1 protein. Cell. 1985 Jan;40(1):139–145. doi: 10.1016/0092-8674(85)90317-4. [DOI] [PubMed] [Google Scholar]
  12. Kmiec E. B., Holloman W. K. Synapsis promoted by Ustilago rec1 protein. Cell. 1984 Mar;36(3):593–598. doi: 10.1016/0092-8674(84)90338-6. [DOI] [PubMed] [Google Scholar]
  13. Lancillotti F., Lopez M. C., Alonso C., Stollar B. D. Locations of Z-DNA in polytene chromosomes. J Cell Biol. 1985 May;100(5):1759–1766. doi: 10.1083/jcb.100.5.1759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Leng M. Left-handed Z-DNA. Biochim Biophys Acta. 1985 Aug 21;825(4):339–344. doi: 10.1016/0167-4781(85)90059-4. [DOI] [PubMed] [Google Scholar]
  15. Maio J. J., Brown F. L., Musich P. R. Toward a molecular paleontology of primate genomes. I. The HindIII and EcoRI dimer families of alphoid DNAs. Chromosoma. 1981;83(1):103–125. doi: 10.1007/BF00286019. [DOI] [PubMed] [Google Scholar]
  16. Malfoy B., Hartmann B., Macquet J. P., Leng M. Immunochemical studies of DNA modified by cis-dichlorodiammineplatinum(II) in vivo and in vitro. Cancer Res. 1981 Oct;41(10):4127–4131. [PubMed] [Google Scholar]
  17. Malfoy B., Rousseau N., Leng M. Interaction between antibodies to Z-form deoxyribonucleic acid and double-stranded polynucleotides. Biochemistry. 1982 Oct 26;21(22):5463–5467. doi: 10.1021/bi00265a013. [DOI] [PubMed] [Google Scholar]
  18. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  19. Nordheim A., Lafer E. M., Peck L. J., Wang J. C., Stollar B. D., Rich A. Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding. Cell. 1982 Dec;31(2 Pt 1):309–318. doi: 10.1016/0092-8674(82)90124-6. [DOI] [PubMed] [Google Scholar]
  20. Pulleyblank D. E., Haniford D. B., Morgan A. R. A structural basis for S1 nuclease sensitivity of double-stranded DNA. Cell. 1985 Aug;42(1):271–280. doi: 10.1016/s0092-8674(85)80122-7. [DOI] [PubMed] [Google Scholar]
  21. Rich A., Nordheim A., Wang A. H. The chemistry and biology of left-handed Z-DNA. Annu Rev Biochem. 1984;53:791–846. doi: 10.1146/annurev.bi.53.070184.004043. [DOI] [PubMed] [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shampay J., Szostak J. W., Blackburn E. H. DNA sequences of telomeres maintained in yeast. Nature. 1984 Jul 12;310(5973):154–157. doi: 10.1038/310154a0. [DOI] [PubMed] [Google Scholar]
  24. Singer M. F. Highly repeated sequences in mammalian genomes. Int Rev Cytol. 1982;76:67–112. doi: 10.1016/s0074-7696(08)61789-1. [DOI] [PubMed] [Google Scholar]
  25. Skowronski J., Plucienniczak A., Bednarek A., Jaworski J. Bovine 1.709 satellite. Recombination hotspots and dispersed repeated sequences. J Mol Biol. 1984 Aug 15;177(3):399–416. doi: 10.1016/0022-2836(84)90292-4. [DOI] [PubMed] [Google Scholar]
  26. Smith G. P. Evolution of repeated DNA sequences by unequal crossover. Science. 1976 Feb 13;191(4227):528–535. doi: 10.1126/science.1251186. [DOI] [PubMed] [Google Scholar]
  27. Solomon M. J., Varshavsky A. Formaldehyde-mediated DNA-protein crosslinking: a probe for in vivo chromatin structures. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6470–6474. doi: 10.1073/pnas.82.19.6470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  29. Tchen P., Fuchs R. P., Sage E., Leng M. Chemically modified nucleic acids as immunodetectable probes in hybridization experiments. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3466–3470. doi: 10.1073/pnas.81.11.3466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Thayer R. E., Singer M. F., McCutchan T. F. Sequence relationships between single repeat units of highly reiterated African Green monkey DNA. Nucleic Acids Res. 1981 Jan 10;9(1):169–181. doi: 10.1093/nar/9.1.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Viegas-Péquignot E., Derbin C., Malfoy B., Taillandier E., Leng M., Dutrillaux B. Z-DNA immunoreactivity in fixed metaphase chromosomes of primates. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5890–5894. doi: 10.1073/pnas.80.19.5890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Vinograd J., Lebowitz J. Physical and topological properties of circular DNA. J Gen Physiol. 1966 Jul;49(6):103–125. doi: 10.1085/jgp.49.6.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Vologodskii A. V., Frank-Kamenetskii M. D. Premelting of superhelical DNA: an expression for superhelical energy. FEBS Lett. 1981 Aug 17;131(1):178–180. doi: 10.1016/0014-5793(81)80914-3. [DOI] [PubMed] [Google Scholar]
  34. Walmsley R. W., Chan C. S., Tye B. K., Petes T. D. Unusual DNA sequences associated with the ends of yeast chromosomes. Nature. 1984 Jul 12;310(5973):157–160. doi: 10.1038/310157a0. [DOI] [PubMed] [Google Scholar]
  35. Zarling D. A., Arndt-Jovin D. J., Robert-Nicoud M., McIntosh L. P., Thomae R., Jovin T. M. Immunoglobulin recognition of synthetic and natural left-handed Z DNA conformations and sequences. J Mol Biol. 1984 Jul 5;176(3):369–415. doi: 10.1016/0022-2836(84)90495-9. [DOI] [PubMed] [Google Scholar]
  36. Zimmerman S. B. The three-dimensional structure of DNA. Annu Rev Biochem. 1982;51:395–427. doi: 10.1146/annurev.bi.51.070182.002143. [DOI] [PubMed] [Google Scholar]

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