Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1986 Apr 25;14(8):3527–3538. doi: 10.1093/nar/14.8.3527

Multiple mechanisms generate extrachromosomal circular DNA in Chinese hamster ovary cells.

S W Stanfield, D R Helinski
PMCID: PMC339791  PMID: 3010241

Abstract

Seven cloned small circular DNA molecules from CHO cells were sequenced and examined for the presence of homologies to each other and to a number of other functional sequences present in transposable elements, retroviruses, mammalian repeat sequences, and introns. The sequences of the CHO cell circular DNA molecules did not reveal common structural features that could explain their presence in the circular DNA population. A gene bank was constructed for CHO chromosomal DNA and sequences homologous to two of the seven small circular DNA molecules were isolated and sequenced. The nucleotide sequences present at the junction of circular and chromosomal DNA suggest that a recombination process involving homologous pairing may have been involved in the generation of one, but not the other, of the two circular DNA molecules.

Full text

PDF
3527

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arkhipova I. R., Gorelova T. V., Ilyin Y. V., Schuppe N. G. Reverse transcription of Drosophila mobile dispersed genetic element RNAs: detection of intermediate forms. Nucleic Acids Res. 1984 Oct 11;12(19):7533–7548. doi: 10.1093/nar/12.19.7533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bertelsen A. H., Humayun M. Z., Karfopoulos S. G., Rush M. G. Molecular characterization of small polydisperse circular deoxyribonucleic acid from an African green monkey cell line. Biochemistry. 1982 Apr 27;21(9):2076–2085. doi: 10.1021/bi00538a015. [DOI] [PubMed] [Google Scholar]
  3. Chen E. Y., Seeburg P. H. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA. 1985 Apr;4(2):165–170. doi: 10.1089/dna.1985.4.165. [DOI] [PubMed] [Google Scholar]
  4. Ciliberto G., Castagnoli L., Cortese R. Transcription by RNA polymerase III. Curr Top Dev Biol. 1983;18:59–88. doi: 10.1016/s0070-2153(08)60579-7. [DOI] [PubMed] [Google Scholar]
  5. DeLap R. J., Rush M. G. Change in quantity and size distribution of small circular DNAs during development of chicken bursa. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5855–5859. doi: 10.1073/pnas.75.12.5855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flavell A. J., Ish-Horowicz D. Extrachromosomal circular copies of the eukaryotic transposable element copia in cultured Drosophila cells. Nature. 1981 Aug 13;292(5824):591–595. doi: 10.1038/292591a0. [DOI] [PubMed] [Google Scholar]
  7. Flavell A. J., Ish-Horowicz D. The origin of extrachromosomal circular copia elements. Cell. 1983 Sep;34(2):415–419. doi: 10.1016/0092-8674(83)90375-6. [DOI] [PubMed] [Google Scholar]
  8. Flavell A. J. Role of reverse transcription in the generation of extrachromosomal copia mobile genetic elements. Nature. 1984 Aug 9;310(5977):514–516. doi: 10.1038/310514a0. [DOI] [PubMed] [Google Scholar]
  9. Fujimoto S., Tsuda T., Toda M., Yamagishi H. Transposon-like sequences in extrachromosomal circular DNA from mouse thymocytes. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2072–2076. doi: 10.1073/pnas.82.7.2072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Georgiev G. P. Mobile genetic elements in animal cells and their biological significance. Eur J Biochem. 1984 Dec 3;145(2):203–220. doi: 10.1111/j.1432-1033.1984.tb08541.x. [DOI] [PubMed] [Google Scholar]
  11. Gilbert W. Genes-in-pieces revisited. Science. 1985 May 17;228(4701):823–824. doi: 10.1126/science.4001923. [DOI] [PubMed] [Google Scholar]
  12. Hardison R. C., Printz R. Variability within the rabbit C repeats and sequences shared with other SINES. Nucleic Acids Res. 1985 Feb 25;13(4):1073–1088. doi: 10.1093/nar/13.4.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jones R. S., Potter S. S. Characterization of cloned human alphoid satellite with an unusual monomeric construction: evidence for enrichment in HeLa small polydisperse circular DNA. Nucleic Acids Res. 1985 Feb 11;13(3):1027–1042. doi: 10.1093/nar/13.3.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jones R. S., Potter S. S. L1 sequences in HeLa extrachromosomal circular DNA: evidence for circularization by homologous recombination. Proc Natl Acad Sci U S A. 1985 Apr;82(7):1989–1993. doi: 10.1073/pnas.82.7.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Krolewski J. J., Bertelsen A. H., Humayun M. Z., Rush M. G. Members of the Alu family of interspersed, repetitive DNA sequences are in the small circular DNA population of monkey cells grown in culture. J Mol Biol. 1982 Jan 25;154(3):399–415. doi: 10.1016/s0022-2836(82)80003-x. [DOI] [PubMed] [Google Scholar]
  16. Krolewski J. J., Rush M. G. Some extrachromosomal circular DNAs containing the Alu family of dispersed repetitive sequences may be reverse transcripts. J Mol Biol. 1984 Mar 25;174(1):31–40. doi: 10.1016/0022-2836(84)90363-2. [DOI] [PubMed] [Google Scholar]
  17. Michel F., Lang B. F. Mitochondrial class II introns encode proteins related to the reverse transcriptases of retroviruses. Nature. 1985 Aug 15;316(6029):641–643. doi: 10.1038/316641a0. [DOI] [PubMed] [Google Scholar]
  18. Mossie K. G., Young M. W., Varmus H. E. Extrachromosomal DNA forms of copia-like transposable elements, F elements and middle repetitive DNA sequences in Drosophila melanogaster. Variation in cultured cells and embryos. J Mol Biol. 1985 Mar 5;182(1):31–43. doi: 10.1016/0022-2836(85)90025-7. [DOI] [PubMed] [Google Scholar]
  19. Nevins J. R. The pathway of eukaryotic mRNA formation. Annu Rev Biochem. 1983;52:441–466. doi: 10.1146/annurev.bi.52.070183.002301. [DOI] [PubMed] [Google Scholar]
  20. Ono M., Ohishi H. Long terminal repeat sequences of intracisternal A particle genes in the Syrian hamster genome: identification of tRNAPhe as a putative primer tRNA. Nucleic Acids Res. 1983 Oct 25;11(20):7169–7179. doi: 10.1093/nar/11.20.7169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Riabowol K., Shmookler Reis R. J., Goldstein S. Interspersed repetitive and tandemly repetitive sequences are differentially represented in extrachromosomal covalently closed circular DNA of human diploid fibroblasts. Nucleic Acids Res. 1985 Aug 12;13(15):5563–5584. doi: 10.1093/nar/13.15.5563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  23. Ruan K., Emmons S. W. Extrachromosomal copies of transposon Tc1 in the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4018–4022. doi: 10.1073/pnas.81.13.4018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schindler C. W., Rush M. G. The KpnI family of long interspersed nucleotide sequences is present on discrete sizes of circular DNA in monkey (BSC-1) cells. J Mol Biol. 1985 Jan 20;181(2):161–173. doi: 10.1016/0022-2836(85)90082-8. [DOI] [PubMed] [Google Scholar]
  26. Shiba T., Saigo K. Retrovirus-like particles containing RNA homologous to the transposable element copia in Drosophila melanogaster. Nature. 1983 Mar 10;302(5904):119–124. doi: 10.1038/302119a0. [DOI] [PubMed] [Google Scholar]
  27. Stanfield S. W., Helinski D. R. Cloning and characterization of small circular DNA from Chinese hamster ovary cells. Mol Cell Biol. 1984 Jan;4(1):173–180. doi: 10.1128/mcb.4.1.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stanfield S. W., Lengyel J. A. Small circular DNA of Drosophila melanogaster: chromosomal homology and kinetic complexity. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6142–6146. doi: 10.1073/pnas.76.12.6142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Stanfield S., Helinski D. R. Small circular DNA in Drosophila melanogaster. Cell. 1976 Oct;9(2):333–345. doi: 10.1016/0092-8674(76)90123-9. [DOI] [PubMed] [Google Scholar]
  30. Varshavsky A. On the possibility of metabolic control of replicon "misfiring": relationship to emergence of malignant phenotypes in mammalian cell lineages. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3673–3677. doi: 10.1073/pnas.78.6.3673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  32. Vizard D. L., Yarsa J. Comparison of genomic fragment and clone sequences within a long interspersed repeated sequence of the mouse genome. Nucleic Acids Res. 1985 Jan 25;13(2):473–484. doi: 10.1093/nar/13.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Yamagishi H., Kunisada T., Tsuda T. Small circular DNA complexes in eucaryotic cells. Plasmid. 1982 Nov;8(3):299–306. doi: 10.1016/0147-619x(82)90067-1. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES