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. 1986 May 12;14(9):3945–3956. doi: 10.1093/nar/14.9.3945

Anisotropic overall and internal motions of short DNA fragments.

T Härd, D R Kearns
PMCID: PMC339826  PMID: 3714500

Abstract

Anisotropic motions of DNA fragments in the size range 6-118 base pairs are studied by the steady-state fluorescence polarization of different excitation transitions in the intercalated ethidium cation. Calculated effective tumbling and twisting times are found to be shorter than predicted for overall motions of rigid DNA, indicating that internal motions and/or dye wobbling contribute to the depolarization. The data are consistent with a model where the DNA fragments are considered to be rigid against bending but torsionally flexible, and where the dye can wobble within the intercalated site. We also discuss the possibility of correlated out-of-plane motions of the dye and the DNA bases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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