Effects of the Polo-like-kinase-1-inhibitor BI2536 in squamous cell carcinoma cell lines of the head and neck

Abstract

Inhibition of the Polo-like-kinase-1 (PLK1) has been shown to be effective in a number of solid tumor models. In this in vitro study, we examined the antitumor effect of BI2536, a small molecule inhibitor of PLK1, in squamous cell carcinoma of the head and neck (SCCHN) cell lines. Dose escalation studies were performed with nine SCCHN cell lines using BI2536. Growth inhibitory and proapoptotic effects were measured quantitatively using cytohistology and a Human Apoptosis Array Kit. BI2536 demonstrated a significant antiproliferative and apoptotic activity in all nine SCCHN cell lines investigated (p<0.009). Our results indicate that inhibition of PLK1 by BI2536 leads to an antiproliferative and apoptotic effect in SCCHN cell lines. In vivo and in the clinical setting, the application of BI2536 may support the antitumoral activity of conventional drugs that are in current use and could decrease the systemic toxicity of these drugs.

Introduction

Cancer of the head and neck is one of the most common types of cancer worldwide. Each year more than 600,000 new diagnoses are made worldwide, with the most common sites being the oral cavity, the larynx and the pharynx (1). Although outcomes can differ between tumors of the same stage, the prognosis of patients suffering from head and neck cancer is mainly dependent on the tumor stage, particularly the lymph node status. Besides tobacco and alcohol abuse, an oropharyngeal infection with high-risk human papillomavirus, such as HPV 16 and HPV 18, may lead to the development of a squamous cell carcinoma, particularly of organs of the oral cavity such as the tonsils, tongue and palate (2). Based on the fact that primarily younger patients suffer from HPV-induced cancer of the head and neck, it seems as if high-risk HPV presents an additional risk factor.

Although surgical techniques and conservative drug therapy strategies have become more efficient in the last decades, prognosis of this type of cancer remains poor. As such, finding new efficient treatment strategies with a long-term beneficial effect is a great challenge. Thus, new antineoplastic drugs must be found and evaluated in vitro and in vivo (3). Fine-mapping of the cell-division cycle, particularly the identification of mitotic kinase signaling pathways, offers new opportunities for cancer-drug development. Polo-like kinase 1 (PLK1) is a major mitotic regulator overexpressed in numerous solid and hematopoietic tumors (4). Moreover, PLK1 plays a key role in the cell cycle at the G2/M transition and in mitosis (5) and is a negative prognostic marker in specific human cancer types (6–8). In this study, we show the first results obtained with BI2536, a potent small-molecule inhibitor of mammalian PLK1, that inhibits PLK1 activity at low nanomolar concentrations. The aim of the present study was to systematically investigate the growth inhibitory and apoptotic activity of BI2536 as a single agent in nine squamous carcinoma cell lines.

Materials and methods

Materials

Nine different squamous carcinoma cell lines, mainly of head and neck origin, were tested in this study. A-431 cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). PE/CA-PJ 15, PE/CA-PJ 41, PE/CA-PJ 49 and PE/CA-PJ 34 cells were obtained from ECACC (European Collection of Cell Cultures), and Cal-27 and Kyse-140 cells from DSMZ GmbH, Braunschweig, Germany. CLS-354 and UM-SCC-14C cells were obtained from Cell Lines Service (CLS, Eppelheim, Germany). The fibroblast cell line was a gift from the Department of Dermatology, University Hospital, Frankfurt/Main, Germany.

Methods

BI2536 was provided by Boehringer Ingelheim GmbH, Austria. Squamous carcinoma cell lines were cultivated according to the instructions of the suppliers with antibiotics at 37°C in the cell type-specific medium Quantum 263 with L-glutamine (PAA Laboratories GmbH, Pasching, Austria). Cells were seeded in 96-multiwell plates (1×10⁵ cells/well). Following incubation for 24 h, the cells were treated with BI2536 for 24, 48 and 76 h, respectively. In the experiments described in this study, BI2536 was used in each cell line at a fixed, cell line-specific concentration that had produced maximum growth inhibition in previous systematic investigations in our laboratory. The concentration for the cell lines investigated was 2.5 nmol/l for BI2536, which demonstrated maximal growth inhibition in our previous dose escalation studies (unpublished data). The number of cells was determined by counting cells in a Rosenthal chamber at 24, 48 and 72 h subsequent to treatment. Apoptosis was detected by microscopic cytohistology as well as by a Human Apoptosis Array Kit (R&D Systems, Abingdon, UK) as previously described by other groups (9,10) (Fig. 2).

Figure 2.

Detection of pro-caspase 3 as a typical apoptosis marker by a Human Apoptosis Array Kit. Besides other cell markers, the typical apoptotic marker pro-caspase 3 was detected in nine squamous carcinoma cell lines treated with BI2536. The results obtained when Kyse-140 cells were treated with BI2536 are shown. Pos.co, positive control.

Statistical analysis

Each experiment was carried out in triplicate. For statistical analysis, a Wilcoxon test for matched pairs (dependent samples) was performed using SPSS 19.0 software for Windows. Differences of p<0.05 were considered statistically significant.

Results

Nine different squamous carcinoma cell lines, mainly of head and neck origin, were tested in this study. Following 24 h incubation, the cells were treated with BI2536 for 24, 48 and 76 h, respectively. Compared with the untreated control groups, the PLK1 inhibitor BI2536 had a highly significant antiproliferative and apoptotic effect in all nine tumor cell lines (p< 0.009) (Fig. 1). Apoptosis was detected by microscopic cytohistology as well as by a Human Apoptosis Array Kit (R&D Systems). Additionally, pro-caspase 3 was detected as a typical apoptotic marker (Fig. 2). BI2536 is therefore a potent small-molecule inhibitor of mammalian PLK1 that inhibits PLK1 activity at low nanomolar concentrations leading to an apoptotic effect in nine squamous carcinoma cell lines.

Figure 1.

Effect of PLK1 inhibitor BI2536 in nine different squamous cell carcinoma cell lines versus untreated control. Growth inhibitory effect of the single agent BI2536 at a fixed cell line-specific concentration of 2.5 nmol/l after 72 h in nine different squamous carcinoma cell lines (black columns) is shown. The corresponding untreated tumor cell lines (grey columns) served as controls and were incubated according to the instructions of the suppliers with antibiotics at 37°C in the cell type-specific medium Quantum 263 with L-glutamine. The number of absolute tumor cells in the treated and control cell lines was determined in a Rosenthal chamber at 72 h after treatment or incubation with Quantum 263 (controls), respectively. The mean values of three independent experiments with standard deviation are shown. Single agent differences versus untreated controls are indicated by asterisks. BI2536 inhibited cell proliferation significantly in all nine cell lines treated (p<0.009 versus controls for all nine cell lines).

Discussion

Previously, a growing body of biochemical investigations contributed to the improved characterization of biological tumor behaviour, thereby improving treatment strategies. PLK1 is a marker of cell proliferation that shows an elevated expression in a number of solid tumors (11,12). Previous studies have been able to demonstrate that the level of PLK1 correlates with metastasis in cervical lymph nodes (13). Moreover, there was a significantly higher occurrence of PLK expression in metastatic HNSCC than in non-metastatic tumors. This observation suggests that the determination of PLK expression in primary tumors of the head and neck indicates the metastasizing potential of a tumor (13). Patients could be subdivided by PLK expression into those with better or worse prognosis, suggesting PLK levels as a discriminatory marker within conventional tumor stages (13). Despite the development of multimodal treatment strategies in head and neck cancer disease, the overall 5-year survival rate in stages UICC III and IV remains poor (14). As such, finding new efficient treatment strategies with long-term beneficial effects is necessary. Thus, new antineoplastic drugs that show less toxicity but a high antineoplastic potency should be evaluated in vitro and in vivo (3,15).

The dihydropteridinone derivate BI2536 is a potent small-molecule inhibitor of mammalian PLK1 that inhibits PLK1 activity at low nanomolar concentrations. BI2536 induces G2/M arrest and the formation of abnormal mitotic figures, such as monopolar spindles. Although there are already clinical phase I and II trials conducted in which BI2536 is administered in different tumor entities there remains a need for further in vitro studies focusing on the pharmacological profile of BI2536 (16). In the present study, we systematically evaluated the effect of BI2536 in nine different squamous cell carcinoma cell lines for the first time. Compared with the untreated control groups, BI2536 had a highly significant antiproliferative and apoptotic effect in all nine tumor cell lines. Taken together, based on our promising results, BI2536 may be of great interest in future treatment regimes used for head and neck cancer.

In conclusion, PLK1 is a major mitotic regulator overexpressed in various solid and hematopoietic tumors. In the present study, BI2536, a potent small-molecule inhibitor of mammalian PLK1 demonstrated, when applied as a single agent, a strong inhibition of cell proliferation accompanied by enhanced apoptosis in nine different squamous carcinoma cell lines. Introducing drugs that block PLK1 to head and neck cancer therapy may improve adjuvant or non-surgical conservative treatment of this tumor identity. More studies are required to prove whether there is an enhancing antitumoral effect of BI2536 in combination with docetaxel or cisplatin that are, among other drugs such as paclitaxel and 5-fluorouracil, commonly used in the modern treatment of head and neck cancer. Our results emphasize that targeting the PLK signaling pathway is a potentially noteworthy therapeutic target for the modern treatment of head and neck cancer disease.