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. 1986 May 27;14(10):4239–4251. doi: 10.1093/nar/14.10.4239

Ultraviolet-induced 8,8-adenine dehydrodimers in oligo- and polynucleotides.

F P Gasparro, J R Fresco
PMCID: PMC339858  PMID: 3714475

Abstract

Characteristic fluorescence excitation and emission is induced by either acetone-sensitized 313 nm irradiation of mixtures of 8-bromoadenosine and adenosine or 254 nm irradiation of oligo- and polynucleotides containing adenine neighbors. The acetone-sensitized reaction involves cleavage of bromine from 8-bromoadenosine with activation of C-8, leading to formation of an 8,8-adenosine dehydrodimer. Comparable fluorescence properties arise in the unsensitized photoreaction of dApdA, pdApdA, ApA, poly(dA), poly(A), poly(dA.dT), and poly(dA.U). The previously unidentified adenine ultraviolet photoproduct described by Porschke has been isolated as several variants from solutions of pdApdA and poly(dA) irradiated at 254 nm. Based upon fluorescence spectra and mass spectra, these variants are shown to contain the 8,8-adenine dehydrodimer moiety.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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