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. 1987 Dec 23;15(24):10211–10232. doi: 10.1093/nar/15.24.10211

Characterization of human DNA sequences synthesized at the onset of S-phase.

C Tribioli 1, G Biamonti 1, M Giacca 1, M Colonna 1, S Riva 1, A Falaschi 1
PMCID: PMC339940  PMID: 2827117

Abstract

We developed a method of enrichment for DNA replicated at the onset of S-phase in synchronized human HL60 cells. About 200 such sequences were cloned. The analysis of this selected DNA sample showed that: 1) the cloned DNA fragments derive from a limited number (750-1500) of replicons; 2) there is no extensive homology between different DNA fragments; 3) they are not significantly enriched in highly repeated sequences; 4) they are enriched in snap-back (Cot = o) DNA. The sequence of the longest fragment revealed the presence of numerous signals collected in a few hundred nucleotides: 1) homology with the origin of replication of human Papovaviruses usually associated with potential stem-loop structures; 2) binding sites for known transcription factors and for another nuclear factor; 3) potential binding sites for the chromosome "scaffold".

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Selected References

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