Figure 5. The nonsense suppression phenotype in strains expressing the Sup35ΔQPrP¹⁵² and Sup35ΔNPrP¹⁹¹ fusion proteins is not dependent on Hsp104 function. (A) The suppression of the ade1–14 nonsense allele in LJ14-derived strains expressing either the Sup35ΔQPrP¹⁵² or the Sup35ΔNPrP¹⁹¹ fusion proteins was assessed by plating on to 1/4YPD plates. The left panels are for the parent LJ14 strain with a wild-type HSP104 gene while the right panel are the same strains but carrying a hsp104::kanMX disruption of the HSP104 gene. (B) Confirmation of the absence of Hsp104 in the hsp104::kanMX strains by western blot analysis using an anti-yeast Hsp104 polyclonal antibody. The filter was reprobed with an anti-yeast PGK polyclonal antibody as a loading control.