Fig. 3.

Immunohistochemistry. A maximum intensity projection of a confocal image of a lower-limb skin biopsy from a control subject (a) and Patient 5 (b) immunostained for protein gene product (PGP) 9.5 (red) to demonstrate axons and collagen type 4 (green) to show the basement membrane of the epidermis. There is no difference in the number of intraepidermal nerve fibres (arrows). (c-h) Confocal images of the dermis immunostained for PGP 9.5 [(c, f) red] and myelin basic protein [(d, g) MBP, green]. In Patient 5 (f, g, h), although numerous axons can be seen, there are no myelinated internodes, while in a control subject (c, d, e), numerous myelinated internodes were observed. Scale Bar: 50 μm.