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. Author manuscript; available in PMC: 2013 Jan 11.
Published in final edited form as: Oncogene. 2012 Feb 27;32(2):190–197. doi: 10.1038/onc.2012.43

Figure 5.

Figure 5

Effect of MNK paralysis on responses to rapamycin. A) ANBL-6 MM cells treated +/− CGP (25 uM) +/− rapamycin (100nM) for 48 or 72 hrs. Viable cell recovery then enumerated by trypan blue exclusion. Data represent means+/−SD of 4 separate experiments. The only significant difference (p<0.05) are in the combined CGP/rapamycin-treated groups at both time points (designated by an asterix). B) U266 cells similarly treated with RAP+/−CGP. Data are means+/−SD, n=3. Significant differences (p<0.05) designated by asterix. C) ANBL-6 MM cells stably transfected with shRNA targeting scrambled sequence (control) or MNK1 sequences (1-1 and 1-4) and treated with or without rapamycin (100 nM) for 48 or 120 hrs followed by enumeration of viable cells. Closed circles are control, non-treated cells and open squares are rapamycin-treated. Data represent means+/−SD of 3 separate experiments. *=significantly different from control (non-rapamycin-treated), p<0.05.